Desarrollo de un modelo experimental para el aislamiento y selección de hongos endófitos con actividad entomopatógena en el bosque mediterráneo para el control de plagas.

  1. Benito Delgado, A. 1
  2. Diez Hermano, S. 1
  3. Diez Casero, J. J. 1
  1. 1 Universidad de Valladolid
    info
    Universidad de Valladolid

    Valladolid, España

    ROR https://ror.org/01fvbaw18

    Geographic location of the organization Universidad de Valladolid
Journal:
Cuadernos de la Sociedad Española de Ciencias Forestales

ISSN: 1575-2410,  2386-8368

Year of publication: 2025

Issue Title: V Reunión Grupo de Trabajo de Sanidad Forestal (Albacete, 19-21 junio de 2024)

Issue: 51

Pages: 139-150

Type: Article

DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Cuadernos de la Sociedad Española de Ciencias Forestales

Abstract

A methodology for the identification and evaluation of entomopathogenic endophytic fungi in forest ecosystems is presented in this study, focusing on their potential as biological control agents of forest pests. For this purpose, the model insect Tenebrio molitor is used to evaluate the pathogenicity of the fungi found. Endophytic fungi were isolated from the tissues of several Mediterranean tree species, Quercus ilex, Quercus pyrenaica, Quercus suber and Castanea sativa, and subsequently tested on T. molitor. The entomopathogenic fungi found are compared with Beauveria pseudobassiana. When compared with Beauveria pseudobassiana, native endophyte fungi found causes 100% mortality on T. molitor, with a little higher efficacy Beauveria pseudobassiana has a TL50 of 8 days with the same mortality rate. These findings suggest that native endophytic fungi could be an alternative to control potential pests within the framework of Integrated Pest Management (IPM).

Bibliographic References

  • Alali S., Mereghetti V., Faoro F., Bocchi S., Azmeh F.A. and Montagna M. 2019. Thermotolerant isolates of Beauveria bassiana as potential control agent of insect pest in subtropical climates. PLoS ONE 14(2). https://doi.org/10.1371/journal.pone.0211457
  • Bamisile B.S., Siddiqui J.A., Akutse K.S., Aguila L.C.R. and Xu, Y. 2021. General limitations to endophytic entomopathogenic fungi use as plant growth promoters, pests and pathogens biocontrol agents. Plants 10(10) https://doi.org/10.3390/plants10102119
  • Cheraghi A., Habibpour B., Mossadegh M. and Sharififard M. 2012. Horizontal transmission of the entomopathogen fungus metarhizium anisopliae in microcerotermes diversus groups. Insects 3(3): 709-718. https://doi.org/10.3390/insects3030709
  • Cory J.S. and Ericsson J.D. 2010. Fungal entomopathogens in a tritrophic context. BioControl. 55(1), 75-88. https://doi.org/10.1007/s10526-009-9247-4
  • Deruytter D., Coudron C.L. and Claeys, J. 2021. The influence of wet feed distribution on the density, growth rate and growth variability of Tenebrio molitor. Journal of Insects as Food and Feed 7(2), 141-149. https://doi.org/10.3920/JIFF2020.0049
  • Eski A. and Murat Gezgin M. 2022. Susceptibility of different life stages of Tenebrio molitor (Coleoptera: Tenebrionidae) to indigenous entomopathogenic fungi. Journal of Stored Products Research 98. https://doi.org/10.1016/j.jspr.2022.102008
  • Ganley R.J. and Newcombe G. 2006. Fungal endophytes in seeds and needles of Pinus monticola. Mycological Research. 110(3), 318-327. https://doi.org/10.1016/j.mycres.2005.10.005
  • Gardes M. and Bruns, T.D. 1993. ITS primers with enhanced specificity for basidiomycetes ? application to the identification of mycorrhizae and rusts. Molecular Ecology. 2(2), 113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  • Gómez-Vidal S., Lopez-Llorca L.V., Jansson H.B. and Salinas, J. 2006. Endophytic colonization of date palm (Phoenix dactylifera L.) leaves by entomopathogenic fungi. Micron. 37(7), 624-632. https://doi.org/10.1016/j.micron.2006.02.003
  • Güerri-Agulló B., Gómez-Vidal S., Asensio L., Barranco P. and Lopez-Llorca L.V. 2010. Infection of the Red Palm Weevil (Rhynchophorus ferrugineus) by the entomopathogenic fungus Beauveria bassiana: A SEM study. Microscopy Research and Technique. 73(7), 714-725. https://doi.org/10.1002/jemt.20812
  • Hajek A.E., Elkinton J.S. and Witcosky J.J. 1996. Introduction and spread of the fungal pathogen Entomophaga maimaiga (Zygomycetes: Entomophthorales) along the leading edge of gypsy moth (Lepidoptera: Lymantriidae) spread. Environmental Entomology. 25(5), 1235-1247. https://doi.org/10.1093/ee/25.5.1235
  • Inglis G.D., Enkerli, J. and Goettel M.S. 2012. Laboratory techniques used for entomopathogenic fungi: Hypocreales. Manual of Techniques in Invertebrate Pathology 189-253. https://doi.org/10.1016/B978-0-12-386899-2.00007-5
  • Jaber L.R. and Ownley B.H. 2018. Can we use entomopathogenic fungi as endophytes for dual biological control of insect pests and plant pathogens? Biological Control 116, 36-45. https://doi.org/10.1016/j.biocontrol.2017.01.018
  • Jamunarani G.S., Ramanagouda S.H., Venkateshalu B., Jayappa J., Raghavendra G., Rudresh D.L., Kulkarni M.S., Mahantesha B.N.N. and Gopali J.B. 2022. Isolation and evaluation of indigenous endophytic entomopathogenic fungus, Beauveria bassiana UHSB-END1 (Hypocreales: Cordycipitaceae), against Spodoptera litura Fabricius. Egyptian Journal of Biological Pest Control 32(1). https://doi.org/10.1186/s41938-022-00617-4
  • Karaborklu S., Altin N., Karabörklü S. and Keskin Y. 2019. Native Entomopathogenic Fungi Isolated from Duzce, Turkey and their Virulence on the Mealworm Beetle [Tenebrio molitor L. (Coleoptera: Tenebrionidae)] Native Entomopathogenic Fungi Isolated from Duzce, Turkey and their Virulence on the Mealworm Beetle Tenebrio molitor L. (Coleoptera: Tenebrionidae). Philippine Agricultural Scientist 102, 82-89. https://www.researchgate.net/publication/333447762
  • Kim J.C., Lee M.R., Kim S., Lee S.J., Park S.E., Baek S., Gasmi L., Shin T.Y. and Kim J.S. 2019 Long-term storage stability of Beauveria bassiana ERL836 granules as fungal biopesticide. Journal of Asia-Pacific Entomology 22(2), 537-542. https://doi.org/10.1016/j.aspen.2019.04.001
  • Kim J.C., Lee M.R., Kim S., Lee S.J., Park S.E., Nai Y.S., Lee G.S., Shin T.Y. and Kim J.S. 2018. Tenebrio molitor-mediated entomopathogenic fungal library construction for pest management. Journal of Asia-Pacific Entomology 21(1), 196-204. https://doi.org/10.1016/j.aspen.2017.11.018
  • Lacey L.A. (2017) Microbial control of insect and mite pests: from theory to practice (Lawrence A. Lacey, Ed.) Elsevier.
  • Lacey L.A., Grzywacz D., Shapiro-Ilan D.I., Frutos R., Brownbridge M. and Goettel M.S. 2015. Insect pathogens as biological control agents: Back to the future. Journal of Invertebrate Pathology 132, 1-41. https://doi.org/10.1016/j.jip.2015.07.009
  • Lacey L.A. and Kaya H.K. (2007) Field Manual of Techniques in Invertebrate Pathology. Springer. https://link.springer.com/content/pdf/10.1007/978-1-4020-5933-9.pdf
  • Lerche S., Meyer U., Sermann H. and Buettner C. 2004. Dissemination of the entomopathogenic fungus verticillium lecanii (Zimmermann) Viégas (Hyphomycetales: Moniliaceae) in a population of Frankliniella occidentalis (Pergande, 1895) (Thysanoptera: Thripidae). Communications in Agricultural and Applied Biological Sciences 69(3), 195-200.
  • Lovett B. and St. Leger, R.J. 2018. Genetically engineering better fungal biopesticides. Pest Management Science 74(4),781-789 https://doi.org/10.1002/ps.4734
  • Maistrou S., Paris V., Jensen A.B., Rolff J., Meyling N.V. and Zanchi C. 2018. A constitutively expressed antifungal peptide protects Tenebrio molitor during a natural infection by the entomopathogenic fungus Beauveria bassiana. Developmental and Comparative Immunology 86, 26-33. https://doi.org/10.1016/j.dci.2018.04.015
  • Mann A.J. and Davis T.S. 2021, Entomopathogenic fungi to control bark beetles: a review of ecological recommendations. Pest Management Science 77(9), 3841-3846. https://doi.org/10.1002/ps.6364
  • Nicoletti R. and Becchimanzi A. 2020. Endophytism of lecanicillium and akanthomyces. Agriculture (Switzerland) 10(6) MDPI AG. https://doi.org/10.3390/agriculture10060205
  • Picciotti U., Araujo Dalbon V., Ciancio A., Colagiero M., Cozzi G., De Bellis L., Finetti-Sialer M.M., Greco D., Ippolito A., Lahbib N., Logrieco A.F., López-Llorca L.V., Lopez-Moya F., Luvisi A., Mincuzzi A., Molina-Acevedo J.P., Pazzani C., Scortichini M., Scrascia M. and Porcelli, F. 2023. "Ectomosphere": Insects and Microorganism Interactions. Microorganisms 11(2) https://doi.org/10.3390/microorganisms11020440
  • R Core Team. 2022. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/.
  • Reay S.D., Brownbridge M., Gicquel B., Cummings N.J. and Nelson T.L. 2010. Isolation and characterization of endophytic Beauveria spp. (Ascomycota: Hypocreales) from Pinus radiata in New Zealand forests. Biological Control 54(1), 52-60. https://doi.org/10.1016/j.biocontrol.2010.03.002
  • Shin T.Y., Lee M.R., Kim J.C., Nai Y.S. and Kim J.S. 2022. A new strategy using entomopathogenic fungi for the control of tree borer insects. Entomological Research 52(7), 327-333. https://doi.org/10.1111/1748-5967.12605
  • Vega F.E., Meyling N.V., Luangsa-ard J.J. and Blackwell M. 2012. Fungal Entomopathogens. Insect Pathology, Second Edition 171-220. https://doi.org/10.1016/B978-0-12-384984-7.00006-3. https://doi.org/10.1016/B978-0-12-384984-7.00006-3
  • Vega F.E., Posada F., Catherine Aime M., Pava-Ripoll M., Infante F. and Rehner S.A. 2008. Entomopathogenic fungal endophytes. Biological Control 46(1), 72-82. https://doi.org/10.1016/j.biocontrol.2008.01.008
  • White T. J., Bruns T., Lee S. and Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. Pcr protocols 315-322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Wraight S. P. and Carruthers R. I. 1999. Production, Delivery, and Use of Mycoinsecticides for Control of Insect Pests on Field Crops. Biopesticides 233-270. https://doi.org/10.1385/0-89603-515-8:233 https://doi.org/10.1385/0-89603-515-8:233
Data source: Dialnet