Actividad de los enzimas glutation-dependientes durante la vitrectomíaEfecto de la solución BSS Plus en retina
- Marcos MA 1
- Cordero Y 1
- Manzanas L 1
- Del Nozal MJ 1
- Pastor JC 1
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1
Universidad de Valladolid
info
ISSN: 0365-6691
Ano de publicación: 2002
Volume: 77
Número: 3
Páxinas: 133-138
Tipo: Artigo
Outras publicacións en: Archivos de la Sociedad Española de Oftalmologia
Resumo
Purpose To determine changes in the retinal activity of two enzymes related to the glutathione metabolism (Glutathione synthetase —GSHS— and glutathione reductase —GSSGR—) after vitrectomy using BSS Plus®. Material and methods Forty-five pigmented rabbits were distributed in 6 groups: group I (GSHS control in retinal tissue); Group II (analysis of GSHS at 3 hours post-vitrectomy); Group III (determination of GSHS at 48 hours after vitrectomy); Group IV (GSSGR control in retinal tissue); Group V (measurement of GSSGR at 3 hours post-surgery) and Group VI (study of GSSGR at 48 hours post-vitrectomy). Statistical analysis was done by a parametric test (ANOVA of single factor) (p<0.05). Results The activity of these enzymes was: Group I (n=5, basal GSHS). 2785,63 D.S. 419,51 U/g; Group II (n=4, GSHS at 3 hours). 6053,50 D.S. 2788,84 U/g; Group III (n=4, GSHS at 48 hours). 7424,30 D.S. 997,47 U/g; Group IV (n=10, basal GSSGR). 150,86 D.S. 24,40 mU/mL; Group V (n=7, GSSGR at 3 hours). 212,03 D.S. 53,30 mU/mL; Group VI (n=8, GSSGR at 48 hours). 210,84 D.S. 46,03 mU/mL. Conclusions At retinal tissue, BSS Plus® intraocular irrigating solution increases GSHS activity without modifications of GSSGR levels. It seems to be related to the «novo» synthesis of glutathione.