Radioimmunoassay for melatonin and its application to fowl pineal research
- Agustín Francisco Sigüenza Molina
- J. M. Recio
- Maria Teresa Agapito Serrano
ISSN: 0034-9402
Año de publicación: 1988
Volumen: 44
Número: 2
Páginas: 221-226
Tipo: Artículo
Otras publicaciones en: Revista española de Fisiología
Resumen
A radioimmunoassay for melatonin has been developed after raising anti-melatonin antibodies in rabbit. Melatonin was extracted from serum or pineal gland of chickens (Gallus domesticus). The radioimmunoassay was performed by using 3H-melatonin as tracer. The standard curve covered the range 0.022-0.345 pmol/vial and the KD value for melatonin was estimated at 1.37 x 10(10) l/mol. The antiserum specificity has been analysed, none of the common melatonin analogues influencing this method of melatonin measurement. The intra-assay variability was 7.2% for serum samples and 8.6% for pineal extract. The inter-assay variability for this biological sample was 15.3% and 6.4% respectively.